Objectives Members Publications
Dr. GUO, WOEI-JIUN Assistant Professor
Sugar transport, nanoparticle application, plant molecular biology
Ph.D 2005 Purdue Univ., Interdisciplinary Life Science program
M.Sc. 1999 National Taiwan Univ., Dept. Horticultural Science
B.Sc. 1997 National Taiwan Univ., Dept. Physics
PostDoc 2008-2011 Carnegie Inst., Dept. Plant Biology, Stanford
PostDoc 2005-2008 Inst. of Plant and Microbiology, Academia
- Contact information:
Address: No.1, University Road, Tainan City 701, Taiwan,
Institute of Tropical Plant Sciences
Tel: +886-6-5050635 ext 3040
Sugar transport: sugar is the two key bioenergy for plant growth and development as well as important signals to various physiological events, such as pathogen defense. Thus, allocation of sugar between various tissues or organelles in the cells is tightly regulated. The allocation is mediated by extensive transport through cell membrane via specific transporters to uptake into and efflux outside the cells or organelles. Despite of ample understanding on uptake mechanisms and their regulation, very little is known what the molecular identity of efflux mechanism is. Therefore, the long term goal of my lab is to dissect the efflux mechanisms of sugar across the plant cell membrane and their physiological roles via molecular and biotechnical approaches.
Figure. The path of sugar transport from source to sink, such as roots and seeds. (modified from
Sauer, 2007, FEBS Letters 581:2309-2817)
FRET nano sensors: It has been difficult to investigate how metabolite compartmentalization is regulated because classical destructive analysis lacks subcelluar resolution. The genetically encoded molecular Förster Resonance Energy Transfer (FRET) sensors provide a powerful non-invasive tool to monitor metabolite fluxes in vivo and in a real-time manner. In principle, FRET sensors make use of ligand-dependent changes in protein conformation that is converted into an emission of fluorescence from a pair of donor-acceptor fluorescent proteins, such as eCFP and eYFP. The changes in fluorescence intensities therefore provide a quantitative measure of the binding substrates. My lab will further apply the FRET nanosensors in planta to understand the regulation of sugar and ATP transport.
DNA nanoscissor:With large progress in high throughput genome sequencing for major crop plants, functional genetic studies via genome modification have became critical to identify key regulators for future crop improvement. Nevertheless, technique for robust sequence specific gene regulation is still lacking in plant. Conventional Agrobaterium mediated random mutation or current site-specific homologous recombination and zinc-fingers nucleases approaches are limited to low efficiency, complexity and time-consuming. In this proposal, we intend to develop instant and non-invasive gene-specific silencing technique in plants via the triplex-forming oligonucleotide (TFO) approach and a photoactivated molecular DNA scissor combining the cutting-edge functional nanoparticle as the delivery tool. We will employ the TFO-based DNA scissors into both plant cell and whole plant system and investigate the methods to generate stable mutated plants.
Figure. Strategy of the DNA nanoscissor. a. Specific TFOs were assembled on gold nanoparticles. b. BMPS- hydrazone was then added to form ATLANS c. TFOs recognize the plasmid DNA containing targeted sequences and form the stable binding d-e. when exposed to UV light, the hydrazone creates a double-strand break.
check: Tsai, T.L., et al., Biomaterials, 2010. 31(25): p. 6545-54.